vue - Aseptic Technique

1. How long should I wait after sterilizing the loop if I dip it into liquid culture?
2. Aren't you supposed to sterilize the loop every time you move to a different quadrant? At least that's what we're doing in lab
3. I'm thrilled that I found this video, I will be doing aseptic technique on Friday in lab. This video was easy to follow and demonstrated proper technique! Thank You
4. I like microbiology but I only have one arm. Can I do all these things with hand without creating major contamination of culture such as by lying the lid of petri dishes on the table while holding something else? I can also close the lid quickly. inoculation loop I know I can stand it up in a test tube holder like the one on the video. I took microbiology lab but for the classes, errors and minor contamination weren't taken in consideration. However, I'd like to work in a lab for school to make some money and some of the duties are aseptic techniques. If I contaminate everything, I assume I'll get fired. is closing the lids quickly really important?
5. This is a very good video. It is direct and easy to understand. This will help me a lot with my lab skills. Thank you so much!
6. helpful and easy video for bactrerial inoculation
7. I love your gloves*-*
8. Thank you so much for this video. It will be of a great help in my laboratory class.
9. +angelou debuyan Thank you for your comment. The video is divided into four sections:
      1) transferring bacteria from a liquid culture to a petri plate
      2) transferring bacteria from a liquid culture to a liquid  (test tube)
      3) transferring bacteria from a petri plate to an agar stab culture
      4) transferring bacteria from a petri plate to a liquid (test tube)
   In the last section, the bacteria is transferred from the petri plate to the inoculation loop and from the inoculation loop to the liquid media. The transfer of bacteria has already occurred by the time he flames the loop for sterilization. I hope that clarifies things.
10. In the last part, why didn't he streak the agar with  the needle obtaining a broth? He just dipped it in the broth and sterilized it and the end. I dont understand
11. Helpful, but does why does he keep saying the same thing?
12. 4:29 did he forget to sterilize the mouth of the tube?
13. Nice info.  Reminds me of my microbiology courses in the 90's.
    
    I was really taken back when I started working in a medical micro lab.  Since the sample isn't sterile to begin with, and most samples are rarely kept for more than 3 days, we don't use flame for anything!  No propane, no bunsen burner, no metal loops or stabs, no reusable/auto-claved glassware.
    
    Disposable plastic loops, plastic test tubes, pre-poured/QC'd plastic agar plates... aseptic technique is a research only technique I suppose.
14. Not the aseptic technique I was looking for but cool.
15. I didn't know Shrek did science.
16. it's really helpful.. Thanks before for uploading.. 
17. Anyone else here for GCSE?