vue - Bacterial culture Media

Don't hesitate to comment below if you have any questions or additional phrases Bacterial CultureMediabasics Dr.T.V.Rao MD ,Major Contribution to Culture Media ,Agar - Agar Frau Hesse’scontribution ,Agar – Agar Solid medium is made by adding Agar Agar is obtained from Sea weeds New Zealand agar is more Agar contain long chain poly saccharides.Inoranic salts and protein like substance Melts at 980c and sets at 420c ,Agar - Agar Complex polysaccharide Used as solidifying agent for culture media in Petri plates, slants, and deeps Generally not metabolized by microbes Liquefies at 98°C Solidifies ~42°C Dr.T.V.Rao MD’s ‘e’ learning series ,Media and Culture Media: Nutrients (agar, pH indicators, proteins and carbohydrates) used to grow organisms outside of their natural habitats Culture: The propagation of microorganisms using various media ,Culture media Used to grow bacteria Can be used to: Enrich the numbers of bacteria Select for certain bacteria and suppress others Differentiate among different kinds of bacteria ,Culture and Medium Culture is the term given to microorganisms that are cultivated in the lab for the purpose of identifying and studying them. Medium is the term given to the combination of ingredients that will support the growth and cultivation of microorganisms by providing all the essential nutrients required for the growth (that is, multiplication) in order to cultivate these microorganisms in large numbers to study them. ,Specific Media Defined media are media composed of pure ingredients in carefully measured concentrations dissolved in double distilled water i.e., the exact chemical composition of the medium is known. Typically, they contain a simple sugar as the carbon and energy source, an inorganic nitrogen source, various mineral salts and if necessary growth factors (purified amino acids, vitamins, purines and pyrimidines ,Need for Culture Media It is usually essential to obtain a culture by grwoing the organism in an artificial medium. If more than one species or type of organism are present each requires to be carefully separated or isolated in pure culture. Several organism need the determination of Antibiotic sensitivity pattern for optimal antibiotic selection ,Basic requirements of culture media Nutrients - Energy source - Carbon source - Nitrogen source Mineral salts – Sulphate, phosphates, chlorides & carbonates of K, Mg & Ca. A suitable pH – 7.2 – 7.4 Accessory growth factors - Tryptophan for Salmonella typhi - X & V factors for H. influenzae ,Pouring the Culture Plates ,Petri dish with Media Plate: provide large surface for isolation and observation of colonies Using a sterile loop or a sterile swab streak your sample on the petri plate Important let your sterilized loop cool before you pick up your sample ,Classification of Culture media Based on the consistency:Liquid -- Peptone water, Nutrient broth Semisolid -- Nutrient agar stabsSolid -- Blood agar, Serum agar Based on Oxygen requirement: -- Aerobic medium -- Anaerobic media ,Aerobic Media Simple media Complex mediaMay be Synthetic or Defined Medium - Enriched media - Differential media - Enrichment media - Selective media Semisyntetic Medium - Sugar media - Transport media ,Aerobic media Liquid media - Peptone water(1% peptone +0.5%Nacl + 100 ml water)- Nutrient broth ( peptone water + 1% meat extract Solid media- Nutrient agar (nutrient broth + 2% Agar) Use: To grow non-fastidious microorganisms Simple media- consists of only basic necessities ,Liquid Medium Difficulat to identify all types of organisms Suitable for isolation of bacteria from Blood culturing and water analysis ,Peptone Peptone contain partially digested proteins Proteases Polypeptides Aminoacids Inorganic salts Phosphates Potassium and Magnesium Riboflavin Meat exract called as Lab lemco ,Nutrient Agar Contain 2% ag