vue - Biosafety: H5N1 Highly Pathogenic Avian Influenza Virus Culturing (BSC, BSL-3)
Biosafety of H5N1 Virus Cultivation. This video shows how highly pathogenic avian influenza virus is inoculated on Vero or MDCK cell cultures in a biosafety cabinet (BSC) at biosafety level 3 (BSL-3). The video was taken during a BSL-3 training. The trainee is a skilled virologist who had been working in a BSL-2 laboratory. To make the work safer, the institute built a BSL-3 laboratory. All processes and manipulations had to be upgraded to BSL-3. The worker shows very good skills, but the practices and procedures and some equipment can still be improved (see comments in the video).
Commentaires
-
@brifranz: Filtered tips should be used. In this training session regular tips were used. Flasks must either be decontaminated before removal, but for large numbers this is not a feasible solution. Instead, fleaks can be put on a clean tray or into a clean box. This is the preferred method for instance in veterinary labs with high troughput-
-
Terrible waste handling! How could it be that you pour the waste to regular plastic bag. You should have a waste container for collecting used medium.
-
Thank you! I figured you were using filter tips and deconning the flasks, but I couldn't tell in the video. I appreciate the instructional videos. Keep doing what you're doing!
-
Other questions/concerns: Filtered pipette tips or no? I can't tell. Decon of flasks prior to removal from BS cab?
18Évaluation