vue - Cell Culture Training Video

1. wanted to use this in the class but for its music ......
2. minute 8:44 supernatant aspirated
   minute 9:46 magically media appears in the test tube.
   Sorry I missed the step in between. After aspirating the supernatant do we add fresh media? If so how much ml? Thanks.
3. For those who are not familiar with centrifugation, you may have to ignore the 1,000 rpm recommendation in this video. Depending on the rotor size, that means you may be applying less or more gravitational force than you want to. Potentially, using 1,000 rpm for your centrifuge setup will result in not collecting all or damaging cells in the process. It is more helpful to give parameters for centrifugation in # x g (meaning some number times gravity). x g is always equal no matter what centrifuge you use, whereas rpm is dependent on rotor size and is associated with a specific x g for that rotor size/centrifuge model.
4. What is the name of the piece of classical music playing in this video at 07:30?
5. These people tried good to teach, however, so many things need to improve.
   1. Cell culture hood should be free of these many things inside. This increase the rick of contamination.
   2. Need to cover hair.
   3. Can not touch goggles after covering with gloves.
   4. Can not wipe with cloth or tissue after 70% IPA spray.
   5. Cleaning of BSC is totally wrong. It should be in one direction only that is back to front and top to bottom or left to right. Sequence is left wall first then back wall, then right wall, then bottom, then inner surface of glass and at the and outer surface of glass. It is always good to wipe the BSC with Sterie (disinfectant) before the wipe with 70% IPA.
   6. Make sure everything goes inside the BSC should be sprayed with 70% IPA including T-flask.
   7. Make sure do not put anything on the grill located at the front end of bottom because it interfere the path of air to be enter in the BSC. The air entering in hood should directly go to HEPA filter.
6. Dr. Clean must learn to cover and uncover the tube with one hand to avoid waiting to use both hands to cover it... :/ and some other... many things...
7. Instructions unclear, what is the correct example, Dr. Clean or Dr. Contamination? I would say Dr. Contamination because she is much more happier...
8. Ethanol will not work if you wipe it off with dry cloth... the whole point of sanitizing with ethanol is to WET what you want to sanitise and LET IT DRY! minimum contact time with ethanol is 5 mins. the actual sanitation is taking place WHILE DRYING as ethanol supposed to dehydrate all of the moister from the bacteria
9. imovie...
10. Media Battle? haha jk. I can hear the different accents
11. Dr. Clean adjusted her eye protection with her gloved hand. Since she earlier donned those goggles before gloving up and sanitizing... she just cross contaminated the outside of the container.
12. Am I the only one who finds the Latin music for Dr. Contamination and the classical music for Dr. Clean a little racist?
    But that aside I liked this video.
13. Good video, I found this Dr. contamination funny :D
14. Dr. mothrfkn' contamination!!!!
15. Dr. contamination is awesome!!
16. The mistakes I noticed in this video is not working atthe centre of the hood as this is considered asceptic area due to lamina flow,please rectify that
17. There is one mistake. Never put the cap of bottle same side at which we fit it on bottle but put cap opposite side when we open it. (Media bottle)
18. Great!!
19. Not upto the mark
20. Thanks for the video, I officially begin learning cell/tissue culture tomorrow at a laboratory at Hopkins for skin conversion/regeneration!