vue - Media Prep
Explore our free eLearning modules at ncbionetwork.org/educational-resources/elearning. In order to grow, cells need very specific environmental conditions such as food, energy, proper temperature and humidity. When growing cells in the lab, we have to create these conditions using culture media. Solid culture media is a mixture of agar and nutrients poured into Petri dishes. We can transfer cells to the Petri dishes where they will grow with proper incubation. Sterile technique is required to create culture media so you don't have unwanted microorganisms growing in your culture. In this video, we'll demonstrate the steps for preparing Brain Heart Infusion Agar. It's a general-purpose media used for a variety of bacterial and fungal species including pathogens like Streptococcus and Pneumococcus.
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Huge mistake there - not wearing a hair cap and having loose hair flap around. It might be ok if you're using a laminar flow cabinet, but if you aren't, it's totally wrong.
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thats some fancy equipment...ill use my range top and a spoon
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like it very nice and easy work 🌸
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That's a lot of effort for 3 dishes.
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thanks for help but somebody tell me more details
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opening the petri dishes widely will cause contamination. The procedure did not perform aseptic technique during the dispensing.
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If the specimens are incubated for too long, the agar can dry out and the organism will likely die.
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Why are agar plates (with micro-organisms on) only incubated for between 24 and 48 hours?
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MANY THANKS
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interesting the advice on avoiding condensation in the petri dishes
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for pouring purpose use laminar air flow. not in normal desk.
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