Mushroom Adventures Episode 11 Part 1 -- Making and Using Spore Prints

vue - Mushroom Adventures Episode 11 Part 1 -- Making and Using Spore Prints

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HEPA filter with laminar: http://fungi.com/shop/mushroom-cultivation-tools/laboratory-equipment-supplies/tools-for-air-filtration.html All American 30qt Pressure Cooker (holds 4 bags): http://www.amazon.com/All-American-30-Quart-Pressure-Cooker-Canner/dp/B0002808Z2/ref=sr_1_3?ie=UTF8&qid=1360879079&sr=8-3&keywords=all+american+pressure+canner All American 41qt Pressure Cooker (holds 6 bags): http://www.amazon.com/All-American-2-Quart-Pressure-Cooker-Canner/dp/B0002808ZM/ref=sr_1_4?ie=UTF8&qid=1360879079&sr=8-4&keywords=all+american+pressure+canner -Dayton 1UHG3 Humidifier Control, Plug In, 120 V: http://www.amazon.com/gp/product/B001OLVNUK/ref=oh_details_o00_s00_i00 -16" Plastic tubing: http://www.ebay.com/itm/290784804734?ssPageName=STRK:MEWNX:IT&_trksid=p3984.m1439.l2649 -Hotpod inline heater: http://www.heater-store.com/hotpod_6_inch_tpi_12719_prd1.htm -6" Hydrofarm inline fans: http://www.ebay.com/itm/6-Inline-Fan-Active-Air-Hydrofarm-400-CFM-Duct-Exhaust-Blower-ACDF6-New-in-box-/190731782721?pt=LH_DefaultDomain_0&hash=item2c68800a41 -Ultrasonic pond foggers: http://www.mainlandmart.com/foggers.html -King oyster culture syringe: http://www.mycopath.com/pleurotus-eryngii-king-oyster-mushroom-p-59.html -Spawn bags: http://www.fungi.com/product-detail/product/sterilizable-airflow-spawn-bags-set-of-100.html

Commentaires

Nichrome wire works great for inoculating loops. its designed to get hot fast and cools pretty quick as well. old toaster ovens use them, straighten the wire and form it to shape. good to go.

Thanks for all the tutorials I really appreciate it. Please provide instructions on the spore printing

destroying tyvek mailers is a federal felony btw, you might want to try tyvek sleeves or elbow patches from a whole suit. I can usually get a leftover roll of tyvek sheeting for housing from contractors or framing crews. otherwise it's like $200 a roll. peace

Hi.How we canned the oyester mushroom?

Excellent information. Many thanks to you for this fine video, I am having immediate success with oysters I am growing.

NOTE: you can buy ANY TYPE OF SYRINGE at your local pharmacy you just need to be 18 or older. and you need to know the length and gauge of the needle and how much volume it can hold. for example you can buy about 100 30g 1/2" 1cc (1 ml) syringes for about 15 dollars, its super cheap. bigger syringes will be more expensive tho

what are the rates of success when not using a fumehood?

Any link about your friend in Greece on what's he doing? we are in greece too, in athens. There is many mushroom farmers at industrial scale for at least 20 years here. But smaller scale, like you, I don't know other project than us : https://www.facebook.com/themushroomcircle

Lol @ only mushroom farmer in Greece. Racist.

I am loving the intro.

wow i came here to learn how to make a spore print not watch a bunch of bullshit. im guessing the info is in here due to the likes/dislike ratio, but i just dont care about wading through 14 minutes of pure shit to look for the raw information. thanks for trying though.

I`m confused. Is there anything in the jar other than the spores that you inject in? Do the spores simply grow inside of the jar without anything to grow on?

Who else is here to learn how to grom magic mushrooms?

So you are adding the spores into the LC you made in your earlier video which composed of yeast, glucose, screws, and water? Or are you adding the spores to a different mixture?

Hi there!
It's my first time viewing your videos, and I'd like to say I am impressed by your actions to maintain a aseptic environment. A quick suggestion to help improve the reliability of the aseptic environment is, at 10:04 do not place the needle cap on the apical surface of a table as many things fall out of the environment that could possibly contaminate the needle (even though your environment is most likely aseptic, it is a good practice nonetheless). Be sure to hold the cap between your pinky finger and your ring finger in the proximal phalanx region. This is a technique taught by my microbio lab professor. Just a suggestion for the future.
Thanks for reading!

Hey my Bro :D i wanna to tell me if the steam has got power to give up the foil and broke the valve so will explode , i ask you , because i am working with your gift tool :) and i am using only jar+grain+filter nothing else :D The steam maybe gimme problems with the foils if i put inside my pressure coocker ? Thank You Bro :)

Why not make the spore prints straight into the shot glass, then add water? Would save a lot of faffing about.

Please make a video on the Petri Dishes with agar mix. Also I did not see in the video how you make the spore print on the aluminum foil. Not sure when you use that technique and for what. loving the vids... thanks

A common misconception among beginners learning sterile technique is that 91% Isopropanol is more effective at killing bacteria than 70% Isopropanol. In fact, it is less effective. More water is needed with the Isopropanol to break the cell wall of the bacteria that you are trying to kill. I see he's using 91% in the video, that's the only reason that I commented. But if it's all you have in the house then it's fine.

I dont think I can 100% agree with with this guys sterile tecnique but still a good video, what ever works I guess.