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This is the first step to actually growing oyster mushrooms (and many other kinds of mushrooms) - making oyster mushroom spawn, or, in other words growing oyster mycelium on grain. In this video I explain the entire process of creating Oyster mushroom grain spawn. This method will also work with many other types of mushrooms if you are working with a live mushroom. You can also inoculate the grain with spores from a spore print, a spore syringe, or from mycelium growing on agar, or a liquid mycelium culture. This method is very low tech, something anyone with a kitchen should be able to accomplish. I have successfully used this method to grow Oyster, Shiitake, Lion's Mane, and a handful of other, edible and medicinal mushrooms. For Lion's Mane and other wood loving species I use hardwood sawdust instead of grain. Getting the moisture level right in sawdust can be a little tricky, but otherwise the process is virtually identical. I mainly just grow Oyster now because it is so easy and quick, and because I have sold or given away a lot of my lab and growing supplies as I am preparing to move to the Philippines and can't take much with me. For each Quart jar I will be filling I use: 1.25 cups of dry grain (wheat or rye) 1/8 tsp gypsum 1 tsp spent coffee grounds. Put the grain, gypsum and grounds in a pan so that it is no more than half full (as the grain will expand) and fill it with hot tap water Soak for 12 to 24 hours Bring to a boil and boil for 10 minutes Drain in a strainer, shake and allow to cool and evaporate the surface moisture away Load jars no more than 3/4 full Put lids on jars and cover with foil to prevent vents from getting wet Boil for 1 hour or Pressure cook @15 PSI for 90 minutes turn off heat and allow to cool for at least 4 hours before inoculating. Here's a list of grains and other media that work well for making spawn: Wheat Rye Rye grass seed (make sure it does NOT have a fungicide in it) Wild bird seed mix Millet Popcorn Hardwood sawdust or chipped (pieces smaller than 1/2") from Oak, maple, walnut, alder or fruit trees, (no pine, fir or hemlock) cotton seed husks Lower nitrogen manure (i.e. horse, cow, goat, deer, goat) when mixed 1:4 with chopped straw or coconut husk fiber