Streak Plate Method - Amrita University

vue - Streak Plate Method - Amrita University

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▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=73&sim=213&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu The most common method of separating bacteria cell on agar surface to obtain isolated colonies is the streak method to inoculate Petri dish. It provides a simple and a raid method of diluting the sample by mechanical means. As the loop is stretched across the agar surface more and more bacteria are separated until the required bacteria are deposited on the agar. After inoculation, the area at the beginning of the streak pattern with show confluent growth while the area at the end of the pattern should show the discreet colonies.

Commentaires

So it doesn't matter how many times you dilute it as long as you get isolated colonies?

thanks <3

it is a very informative video

The lack of isolated colonies on the agar plates in this video show that this is not a good demonstration of proper streaking technique.

thank u vry much

Thanks very much. I was looking for something simple and to the point for my high school student, but this could easily by used as a quick review for first year university students too.

thank you very much

this is very useful for students

nice .. very useful

i really can't thank the author enough.......need i say? it was very helpful "to me"...yeaaa

I gt 1 colony from the plate and find out tat colony is gram negative bacteria, does it means that all the colonies in that plate r gram negative?

thank you so much for the video..very helpfull

nice

amazing video...thank u alot...u made microbiology to easy and interesting to learn :)

طريقة زراعة البكتريا على أطباق الاجار 1 Plate streaking technique 1

Great video: very clean, organized and easy to understand. Thank you!

I would think the streaking techque is incorrect. The point of streaking is reduction of the organisms via several streaks. Each successive streak having fewer and fewer organisms. Hopefully, you'll be able to isolate individual colonies and work with them.

I conditionally disagree with you. Read my reply to Debra Tumboe.

Yes, under most circumstances. The aerobes will grow on the surface and any anaerobes that may have been present may show growth under the agar. I'm guessing you streaked to hard on the plate and dug into the agar, yes? You'll get your technique down, don't worry. BTW, some agar plates are firmer or softer than others, learn what to expect when working with various agar plates. Also, there are many, many types of agar plate formulae but usually you'll only be working with a few of them.

if it broke it wont grow well